The Recognition and Determination of Double-Stranded DNA Using Amikacin by Resonance Rayleigh Scattering Method (CAT#: STEM-ST-0035-YJL)

Introduction

Nucleic acid is a sort of important biological macromalecule, and the quantification of nucleic acids, in particular double-stranded (ds) DNA, is crucial in many molecular biological and diagnostic applications. These include measurements of nucleic acids in biological material, determinations of cell concentration or cell proliferation, as well as the pre-, online-, or postevaluation of polymerase chain reactions (PCR).
Amikacin (AK) is a semisynthetic, water soluble, broad spectrum aminoglycoside antibiotic. It is commonly administered parenteral for the treatment of Gram-negative infections resistant to gentamicin, kanamycin or tobramycin because the AK molecule has fewer points susceptible to enzymatic attack than most other aminoglycosides. However, it has been well known that they could cause damage to the kidneys and cranial nerves. Therefore, it is important to study the reaction of AK with nucleic aicd.