The Study of the Interaction of BleomycinA5 with Nucleic Acids by Resonance Rayleigh Scattering Method (CAT#: STEM-ST-0033-YJL)

Introduction

There are many anticancer drugs used clinically that can destroy DNA's structure, affect the gene control function and gene expression of DNA through binding with DNA in cancerous cells and demonstrating their anticancer activation. Therefore, it is very important to study the interaction of DNA with anticancer drugs not only for elucidating the action mechanisms of some antitumour drugs, but also for designing the new-type of anticancer drugs. BleomycinA5 (BLMA5) belongs to a family of glycopeptide-derived antitumour originally isolated from cultures of Streptomyces verticillus. It is one of the bleomycins antibiotics, which has more activity, lower toxicity and is widely used for the clinical treatment of a variety of cancers, notably squamous cell carcinomas, testicular tumors and malignant lymphomas.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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