Study of the Interaction of Nucleic Acids with Acridine Red and CTMAB by a Resonance Light Scattering Technique (CAT#: STEM-ST-0034-YJL)

Introduction

The qualitative and quantitative analysis of nucleic acids as the material base of genetic inheritance is becoming more and more important. Especially in the quantitation, because their natural fluorescence intensity is very weak, their fluorescence spectra have scarcely been used to study their biological properties, and usually some fluorescence probes have been employed for the investigation.
Acridine red is a cationic tricyclic heteroaromatic fluorescent dye, which forms a dimer in the presence of an appropriate amount of cationic surfactant sodium dodecylsulfonate, and its self-polymerization equilibrium is destroyed with the addition of protein. CTMAB is a cationic surfactant. It is mainly used as a fungicide, softener, emulsifier and antistatic agent in cosmetics.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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