Immunoassay Detection Using Functionalized Gold Nanoparticle Probes Coupled with Resonance Rayleigh Scattering (CAT#: STEM-ST-0057-YJL)

Introduction

Highly sensitive detection and accurate analysis of specific target molecules in human serum samples are important for early clinical diagnosis and treatment. Significant efforts have been made in recent years to apply nanoprobes to realize highly sensitive bioassay, such as the detection of DNA sequence, antibody, and antigen. Nanoprobes initially are fabricated by a sequential self-assembling of functional molecules and specific antibodies on nanoparticle surface.
Gold nanoparticles are known to have large light absorption and scattering cross section in the surface plasmon resonance wavelength regions. The magnitude of light scattering by gold nanoparticles can be orders of magnitude higher than light emission from fluorescent dyes. These unique properties have many important and promising applications in the biomedical field, such as molecular imaging, biosensing, and bioassays.