Study of the Interaction of Heparin with Some Basic Phenothiazine Dyes by Resonance Rayleigh Scattering (CAT#: STEM-ST-0043-YJL)

Introduction

Heparin (Hep) is a polysaccharide belonging to the glycosaminoglycan family. It exists in the blood excreted by hypertrophy cells of the body. It has a variety of biological activities such as anticoagulant, antilipemic, antithrombotic, immunoregulatory, antiphlogistic and antianaphylactic activities, etc. It is the most important medicine to prevent thrombosis and to cure urgent vein thrombus. In recent years, investigations have shown that the efficacy of the heparin correlates intimately with its dosage. Therefore, studying the dose–effect relationship of heparin and finding out its optimum dosages for different diseases will expand the application of this medicine in clinical therapy.
Methylene blue (MB), Azure B and toluidine blue (TB) are basic phenothiazine dyes, which are mainly used as biological coloring agents and spectrophotometric color reagents in ion association complexes. It has been discovered in recent years that the spectral properties of MB are similar to those of blood porphyrin and it may be used in cancer treatment as a photosensitizer. Therefore, there is an important theoretical value to study the interaction of phenothiazine dyes such as MB with biological macromolecules such as heparin.