The Study of the Reaction of Nucleic Acids with Thionine by Resonance Rayleigh Scattering Method (CAT#: STEM-ST-0031-YJL)

Introduction

Nucleic acid is a sort of important biological macromalecule, and its quantitative analysis is crucial in many molecular biological and diagnostic applications. These include measurements of nucleic acids in biological material, determinations of cell concentration or cell proliferation, as well as the pre-, online-, or postevaluation of polymerase chain reactions (PCR). Generally, the conventional spectrophotometric method used nucleic acids assay is based on the measurement of ultraviolet absorption at 260 nm.
Phenothiazine dyes are photocytotoxic, and can cause photoinduced mutagenic effects. In living system, DNA acts as an important target for phenothiazine dyes. It has proved that these dyes can photosensities biological damage. Therefore, it is important to study the interaction of phenothiazine dyes with nucleic acids. Thionine (TH) is an easy available cationic phenothiazine dye and often used as biological staining agent.