Adenoviral Transduction (CAT#: STEM-GT-0006-WXH)

Introduction

The term "transduction" is used to describe a virus-mediated transfer of nucleic acids into cells. In contrast to transfection of cells with foreign DNA or RNA, no transfection reagent is needed here. The viral vector, itself, also called virion, is able to infect cells and transport the DNA directly into the nucleus, independent of further actions. After the release of the DNA into the nucleus, the protein of interest is produced using the cells' own machineries.<br />Adenoviral vectors have proven to be a very successful transduction tool in many eukaryotic cell types, such as human and rodent cells. Besides dividing cell lines, this method gives access to difficult-to-transfect cells, such as primary cells. Adenovirus-mediated transduction is always transient, meaning that no nucleotide integration into the host genome occurs. Transduction efficiencies of up to 100% can easily be achieved. Importantly, biosafety level S2 is needed for adenoviral transduction.