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Membrane fusion for transfection (CAT#: STEM-GT-0001-WXH)

Introduction

Membrane fusion is a novel and highly superior transfection method to incorporate various molecules and particles into mammalian cells. Not only cells that undergo cell division, but also primary and non-dividing cells can be transfected by using this innovative method. It allows for highly efficient transfer of mRNA, siRNA, proteins, beads, dyes, and other molecules. Since the reagents are non-toxic to sensitive and difficult-to-transfect cells, even primary neurons, keratinocytes, and stem cells retain high viability after the transfection. Additionally, the very short incubation times minimize cell stress and provide an almost unaffected cell behavior after the molecule transfer.




Principle

Liposomal carriers, which consist of neutral and cationic lipids, are used to transfer the molecule of interest via membrane fusion into the cell. The molecules are first incorporated into the liposomal carriers, which upon contact, instantly fuse with the cell membrane. The included molecules are then directly released into the cytoplasm without processes such as endocytosis and lysosomal degradation. This results in fusion efficiencies up to 80–100% within only seconds to a few minutes, depending on the cell type.

Applications

Efficient transfer of mRNA, siRNA, proteins, beads, dyes, and other molecules.

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