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Membrane fusion is a novel and highly superior transfection method to incorporate various molecules and particles into mammalian cells. Not only cells that undergo cell division, but also primary and non-dividing cells can be transfected by using this innovative method. It allows for highly efficient transfer of mRNA, siRNA, proteins, beads, dyes, and other molecules. Since the reagents are non-toxic to sensitive and difficult-to-transfect cells, even primary neurons, keratinocytes, and stem cells retain high viability after the transfection. Additionally, the very short incubation times minimize cell stress and provide an almost unaffected cell behavior after the molecule transfer.