Condition: Certified pre-owned
Optics
Laser configuration: Spatially separated beams with 10 × 80 μm elliptical spots
Optical alignment procedure: Fixed, no operator alignment required
Flow cell: 170 × 290 μm rectangular quartz flow cell
Scatter resolution: 0.2 μm
Cell size: 0.2 to 50 μm
Fluorescence threshold sensitivity: FITC <75 MESF, PE <50 MESF, APC <20 MESF
Fluorescence resolution: <3% CV for CEN
Filters: User exchangeable
Fluidics
Sample acquisition rate: 35,000 events/second
Volumetric absolute count precision: Syringe pump: CV <5%
Sample flow rate: 5 to 120 μL/min
Sheath flow rate: 6.5 mL/min
Sample aspiration volume: 10 μL to 5 mL
Fluid container capacity: 3 L sheath, 3 L waste, 500 mL cleaning, 500 mL decontamination
Carryover: <0.1%
Fluidics maintenance: Automated startup, cleaning, decontamination, and shutdown
Data processing
Parameters: Height and area for FSC, SSC and all fluorescence channels, width, and time
Dynamic range: 24 bit, 7.2 decades logarithmic scale, no need for PMT voltage adjustment
Compensation: Automatic compensation, manual compensation, visual compensation tools available for pre/post/live acquisitions
Output data files: FCS 3.1, NovoExpress (.ncf), PDF reports, bitmap graphics, vector graphics, CSV
Workstation: Dell OptiPlex 7040 SFF, 1 TB with 23.8 in LCD monitor
Computer operating system: Microsoft Windows 7 Professional (64 bit), Microsoft Office 2016
Software: Agilent NovoExpress
Sampling
Manual sample loading: 12 × 75 mm tube, 1.5 mL Eppendorf tube
Automatic sample loading: Optional – compatible with 12 × 75 mm tube, 1.5 and 2 mL tubes, "bullet" tubes in 96-position racks, 24-well, 48-well, and 96-well microtiter plates
Operating conditions
Instrument dimension (W × D × H): 23.6 × 17.7 × 15.4 in (60 × 45 × 39 cm)
Instrument weight: 86 lb (39 kg)
Power requirements: 100/115/230 VAC, 50 to 60 Hz
Environment requirements: Temperature: +15 to +32 °C, relative humidity: 80% maximum