BD Biosciences 2009 Fortessa Cell Analyzer - 3 Laser (R/B/V) with HTS, BD Biosciences (CAT#: STEM-CAPO-0028-ZJF)

The BD Biosciences 2009 Fortessa Cell Analyzer - 3 Laser (R/B/V) with HTS is manufactured by BD Biosciences.
The BD LSRFortessa™ Cell Analyzer offers the ultimate in choice for flow cytometry, providing power, performance and consistency.
Designed to be affordable and expandable, the BD LSRFortessa™ System has the flexibility to support the expanding needs of multicolor flow cytometry assays.
The system is an innovative and proven platform for multicolor analysis with its powerful fluidics systems and novel optics design, all of which work to maximize signal and enable minimum cross talk.

Condition: Certified pre-owned
Excitation Optical Platform:
The BD LSRFortessa optical layout allows for up to four lasers.
Laser Power:
355 nm: 20 mW
405 nm: 50 mW
488 nm: 50 mW
640 nm: 40 mW
Optical Efficiency:
Power loss at flow cell: <20% of specified laser power
Performance
Fluorescence Sensitivity:
FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
PE-Cy™5: 10 molecules of equivalent soluble fluorochrome (MESF-PE-Cy™5)
APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A)
PE-Cy5 and APC measurements performed using SPHERO Ultra Rainbow Calibration Particles (URCP-38-2K)
Fluorescence Resolution:
Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
Fluorescence Linearity:
Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
Forward and Side Scatter Sensitivity:
Enables separation of fixed platelets from noise.
Forward and Side Scatter Resolution:
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Side Scatter Resolution:
Enables separation of 0.5-µm beads from noise.
Forward Scatter PMT Option:
A forward scatter PMT upgrade is available for small particle detection through the special order program.
Data Acquisition Rate:
40,000 events/sec with beads.
Warranty: 90-day warranty

The BD LSRFortessa™ System’s innovative excitation and collection optics design reduces excitation losses and optimizes collection efficiency for increased sensitivity and resolution.
The BD LSRFortessa™ System’s fluidics system features a true fixed alignment flow cell that is gel-coupled to the collection optics to maximize detector signal.
The BD® High Throughput Sampler (HTS) option improves experimental workflow and the BD Special Order Research Product (SORP) program provides flexibility.

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