Analysis Affinity of Single-Chain Variable Fragment (scFv) Antibodies by KinExA (CAT#: STEM-MB-0037-CJ)

Introduction

Single chain variable fragment (scFv) is not an antibody fragment, but a fusion protein of immunoglobulin heavy chain (VH) and light chain (VL) variable region, which can be connected by a short connector to a peptide of ten to about twenty-five amino acids. The joint is usually rich in glycine for flexibility, and serine or threonine for solubility, and can connect the N terminal H of V to the C terminal of V L. As an alternative, SCFVS can be created directly from the Ac grand and light chains derived from hybridoma. SCFVS have many uses, for example, in flow cytometry, immunohistochemistry, and as ant+E129igen binding domains for artificial T cell receptors (chimeric antigen receptors). Unlike monoclonal antibodies, which are usually produced in mammalian cell cultures, SCFVS are typically produced in bacterial cell cultures such as E. coli.