Analysis Immunoassay of β-Subunit of Human Chorionic Gonadotropin (β-hCG) by KinExA (CAT#: STEM-MB-0071-CJ)

Introduction

β-HCG is a glycoprotein presumed to be produced by placental syncytiotrophoblastic cells and composed of alpha and beta subunits. The value of β-hCG kinetics lies in the distinction between normal and abnormal pregnancies, including ectopic pregnancies. In ectopic pregnancy, inadequate decidua of the fallopian tube leads to delayed growth and development of trophoblast tissue and delayed production of beta hCG. In addition, elevated beta-hCG indicates the presence of syncytiotrophoblast cells, as seen in choriocarcinoma, seminoma or asexual tumor, and occasionally embryonic carcinoma.

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Principle Applications Procedure Materials Notes Related Products

Principle

KinExA is a two-stage analysis system. In the first stage, a number of solutions are prepared, where one partner remains constant (constant binding partner, or CBP) and the other (titrant) is variable, usually serially diluted. As the titrant is added, the free CBP decreases and is analysed by a sophisticated and precise microfluorescence measurement device. The signal generated can be mathematically related to the affinity (KD) of the two molecules for each other, as well as the kinetic parameters of binding (kon) and dissociation (koff).

Applications

Immunology/Inflammation, Toxicology

Procedure

1. preparation of the functionalized beads which will capture the analyte for measuremen.
2. preparation of a series of solutions consisting of a constant initial concentration of one component of the binary reaction and serial dilutions of the other reactant. The component that is kept constant is the constant binding partner (CBP) , and is the one which will be analyzed.
3. each reaction mixture is sampled and the fluorescence of free CBP bound to the capture beads is obtained for subsequent numerical analysis.

Materials

• Sample Type: Serum, Plasma, Urine
• Equipment: Kinetic Exclusion Assay (KinExA)

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