Analysis Interactions of AhR-Ligand by Microscale Thermophoresis (MST) (CAT#: STEM-MB-2450-LGZ)

Introduction

The aryl hydrocarbon receptor (AhR) is a highly conserved cellular sensor that detects a variety of environmental pollutants and dietary, cellular and microbial metabolites, playing important roles in fundamental biological processes. Disruption of the AhR pathway is associated with a variety of diseases, including autoimmune diseases and cancer, making AhR a promising target for drug development and host-directed therapy. Pharmacological intervention of AhR processes requires detailed information on ligand binding properties to allow specific targeting of specific signaling processes without affecting other signaling processes. We use microscale thermophoresis (MST) to monitor the binding of purified recombinant human AhR to its native ligand in a cell-free system. This approach facilitates the precise identification and characterization of unknown AhR ligands and provides a screening strategy for the discovery of potential selective AhR modulators.