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Microscale Thermophoresis (MST) is a powerful novel fluorescence technique capable of measuring biomolecular interactions and binding affinity (KD). MST is an immobilization-free alternative to surface plasmon resonance (SPR), which is less costly than isothermal titration calorimetry (ITC). MST can be used to determine DSP-lipid interactions, as well as accurate measurement of KD under physiologically relevant solution conditions.