Analysis of ACBD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0747-LGZ)

Introduction

Official Full Name: acyl-CoA binding domain containing 3<br />Also known as: PAP7; GCP60; GOCAP1; GOLPH1<br />The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Turkey Astrovirus (TAstV) by Real-Time PCR Method (CAT#: STEM-MB-3665-LGZ)
Analysis of KCNK12 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1137-LGZ)
Analysis of Riemerella Anatipestifer by Real-Time PCR Method (CAT#: STEM-MB-3544-LGZ)
Analysis of VPS72 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0757-LGZ)
Analysis of TERT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1839-LGZ)
Analysis of Nematodirus Abnormalis by Real-Time PCR Method (CAT#: STEM-MB-4046-LGZ)
Analysis of SNAPIN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0315-LGZ)
Analysis of Canine Adenovirus (CAV) by Real-Time PCR Method (CAT#: STEM-MB-2700-LGZ)