Analysis of Alkeran binding with P53 protein by UV-Vis Spectroscopy (CAT#: STEM-MB-0976-WXH)

Introduction

Alkeran, is a chemotherapy medication used to treat multiple myeloma, ovarian cancer, melanoma, and AL amyloidosis. It is taken by mouth or by injection into a vein.
p53, also known as Tumor protein P53, cellular tumor antigen p53 (UniProt name), or transformation-related protein 53 (TRP53) is a regulatory protein that is often mutated in human cancers. If the DNA can be repaired, p53 activates other genes to fix the damage. If the DNA cannot be repaired, this protein prevents the cell from dividing and signals it to undergo apoptosis. By stopping cells with mutated or damaged DNA from dividing, p53 helps prevent the development of tumors.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer

Other recommended products

Analysis of Protein-Nanoparticle Interactions by UV-Vis Spectroscopy (CAT#: STEM-MB-0926-WXH)
Analysis of Microbial Rhodopsins by UV-Vis Spectroscopy (CAT#: STEM-MB-0995-WXH)
Analysis of interactions of bioactive vanadium species with bovine serum albumin by UV-Vis Spectroscopy (CAT#: STEM-MB-0997-WXH)
Water Quality Analysis by UV-Vis Spectroscopy (CAT#: STEM-MB-0866-WXH)
Analysis of manganese species-protein interactions by UV-Vis Spectroscopy (CAT#: STEM-MB-0961-WXH)
Analysis of Conformational Changes in Albumin by UV-Vis Spectroscopy (CAT#: STEM-MB-0951-WXH)
Assessment of Pearl Quality by UV-Vis Spectroscopy (CAT#: STEM-MB-0900-WXH)
Wine detection and discrimination by UV-Vis Spectroscopy (CAT#: STEM-MB-0868-WXH)