Analysis of Alpha-amylase 1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0019-LGZ)

Introduction

Alpha-amylase 1 is an enzyme encoded in humans by the AMY1A gene. The gene is present in many organisms. Amylase is a secretory protein that hydrolyzes the 1, 4-alpha-glucoside bond in oligosaccharides and polysaccharides, thus catalyzing the digestion of dietary starches and glycogen as the first step.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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