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Analysis of IgM (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0288-LGZ)

Introduction

Immunoglobulin M (IgM) is the immunoglobulin with the largest molecular weight, which is mainly secreted and synthesized by plasma cells in the spleen and lymph nodes, and is divided into two subtypes, IgM1 and IgM2. It is mainly distributed in the serum and exists in the form of pentamer, accounting for 5% to 10% of the total serum Ig. IgM has powerful bactericidal, complement activating, immunomodulatory and agglutination effects, and is also involved in the pathological process of some autoimmune diseases and hypersensitivity reactions.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Assay Development

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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