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Analysis of FABP2/I-FABP (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0160-LGZ)

Introduction

Fatty acid-binding protein (FABP) is a member of the intracellular lipid-binding protein superfamily, present in the cells of vertebrates and invertebrates, and plays an important role in the uptake, transport and metabolic regulation of long-chain fatty acids. The role of FABPs in cells is to promote fatty acid solubilization, transport and metabolism. At least 10 fatty acid binding proteins have been identified, and these fatty acid binding proteins exist in different forms in different tissues.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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