Analysis of HIF-1α (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0254-LGZ)

Introduction

Hypoxia inducible factor-1 (HIF-1) is a nuclear protein with transcriptional activity, which has a wide target gene spectrum, including nearly 100 target genes related to hypoxia adaptation, inflammation development and tumor growth.

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Principle Applications Procedure Materials

Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Goat Polyclonal

Applications

Hypoxia, Phosphoproteins, Cardiovascular, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Whole Cell Lysate

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