Analysis of Sclerostin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0538-LGZ)

Introduction

Sclerostin is a glycoprotein encoded by the sost gene and secreted by osteogenic cells that inhibits bone formation. It can inhibit the signaling pathway by competitively inhibiting the co-receptor-LRP-5 in the Wnt signaling pathway, thereby affecting bone formation.

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Principle Applications Procedure Materials

Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Goat Polyclonal
Detection Antibody: Goat Polyclonal
Recombinant Standards: Recombinant human protein expressed in a mouse myeloma cell line

Applications

Bone Disorders

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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