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Analysis of MIP-4 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0429-LGZ)

Introduction

Macrophage inflammatory protein-4 (MIP-4), also known as CCL18, is a chemokine expressed in lymph nodes, lung, placenta, and bone marrow. Although the receptor for MIP-4s remains unidentified, MIP-4 is known to act as a chemoattractant for both activated and inactivated T cells.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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