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Amino acids (AAs) are the basic units of proteins and they are essential for human health. Tryptophan (Trp), tyrosine (Tyr) and phenylalanine (Phe) play important roles in many biochemical processes. The improper metabolism of Trp can produce toxic substances in brain, which may cause hallucinations, delusions and schizophrenia. Phenylketonuria is a disorder of Phe metabolism and can result in mental retardation and organ damage. The disease is clinically characterized by a remarkable increase in the level of free Phe present in blood. Tyr is the precursor of neurotransmitters in mammalian central nervous systems. The absence of Tyr might cause albinism and alkaptonuria. Thus, detecting these AAs in blood is helpful to diagnose the related diseases. This service provides a method for determination of trace amounts of AAs in bovine blood by combining solid phase extraction (SPE) and capillary electrophoresis (CE).