Analysis of ASCL5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0787-LGZ)

Introduction

Official Full Name: achaete-scute family bHLH transcription factor 5
Also known as: ASH-5; AmeloD; bHLHa47
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Predicted to be part of RNA polymerase II transcription regulator complex and chromatin.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of HNRNPUL2-BSCL2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2453-LGZ)
Analysis of LCE2D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0414-LGZ)
Analysis of Feline Herpesvirus type 1 (FHV-1) by Real-Time PCR Method (CAT#: STEM-MB-3992-LGZ)
Analysis of Campylobacter Laridis by Real-Time PCR Method (CAT#: STEM-MB-2710-LGZ)
Analysis of OR2L5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0523-LGZ)
Analysis of Bovine Parainfluenza Virus Type 3 by Real-Time PCR Method (CAT#: STEM-MB-2883-LGZ)
Analysis of Cortex Phellodendri by Real-Time PCR Method (CAT#: STEM-MB-3949-LGZ)
Analysis of LOC100507346 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1936-LGZ)