Analysis of ATL3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2452-LGZ)

Introduction

Official Full Name: atlastin GTPase 3
Also known as: HSN1F
This gene encodes a member of a family of dynamin-like, integral membrane GTPases. The encoded protein is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. Mutations in this gene may be associated with hereditary sensory neuropathy type IF. Alternatively spliced transcript variants that encode distinct isoforms have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of AARD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1908-LGZ)
Analysis of SRM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1420-LGZ)
Analysis of GSN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1931-LGZ)
Analysis of FCRL6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0979-LGZ)
Analysis of SLC35E2B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1426-LGZ)
Analysis of ADAMTSL4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0772-LGZ)
Analysis of LOC122757938 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1138-LGZ)
Analysis of LNCATV Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0439-LGZ)