Analysis of the expression levels of the mutated BRAFV600E mRNA by RT-qPCR (CAT#: STEM-MT-0045-LGZ)

Introduction

The BRAFV600E gene encodes a mutant BRAFV600E protein that triggers downstream oncogenic signaling in thyroid cancer. As most currently available methods focus on detecting BRAFV600E mutations in tumor DNA, information on BRAFV600E mRNA levels in primary thyroid cancer tumors is limited, and the diagnostic relevance of these RNA mutations is unclear.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Thyroid Cancer

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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