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Analysis of ATP6V1G3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0808-LGZ)

Introduction

Official Full Name: ATPase H+ transporting V1 subunit G3
Also known as: Vma10; ATP6G3
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles in eukaryotic cells. V-ATPase-dependent acidification of organelles is essential for intracellular processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and generation of synaptic vesicle proton gradients. V-ATPase consists of a cytoplasmic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus C, D, E, F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain is composed of 5 distinct subunits: a, c, c', c' and d. Other isoforms of many V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants of this gene have been found to encode different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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