Analysis of ATPase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1469-LGZ)

Introduction

Sensitivity: 0.06 U/L
Detection range: 0.06 - 2.75 U/L
Precision: inter-lot difference of 5%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 630-650 m

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Principle Applications Procedure Materials Notes Related Products

Principle

ATPase catalyzes the hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and inorganic phosphorus, and then determines the activity of the enzyme by determining the content of inorganic phosphorus in unit time by determining the phosphorus method.

Applications

It is used to detect the activity of ATPase in animal tissue and cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissue and cell samples

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