Analysis of Total Cholesterol (TC) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1346-LGZ)

Introduction

Sensitivity: 0.09 mmol/L
Detection range: 0.09-25.85 mmol/L
Precision: inter-lot difference of 2.8%, intra-lot difference of 1.1%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 510 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Total Cholesterol (TC) includes free cholesterol and cholesterol esters. cholesterol ester can be hydrolyzed into cholesterol and free fatty acids by cholesterol esterase (CE), and cholesterol was oxidized in cholesterol oxidase (CO) to produce 4-cholestenone and hydrogen peroxide. When hydrogen peroxide is present in 4-amino-antipyrine and phenol, it is catalyzed by oxidase (POD) to generate red quinone compound benzoquinimide phenazone. The color depth is proportional to the content of TC.

Applications

For the determination of total cholesterol content in serum (plasma) and tissue.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, tissue

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