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Analysis of bio-functionalized nanodiamonds by UV-Vis Spectroscopy (CAT#: STEM-MB-1000-WXH)

Introduction

A nanodiamond is a diamond particle with a size in the nanometer range or one-millionth of a millimeter. Nanodiamonds are made of a diamond core and outer layers of amorphous carbon. The shape, core, and surface are the most critical aspects of the structure of nanodiamonds.
Diamond nanoparticles have the potential to be used in myriad biological applications and due to their unique properties such as inertness and hardness, nanodiamonds may prove to be a better alternative to the traditional nanomaterials currently utilized to carry drugs, coat implantable materials, and synthesize biosensors and biomedical robots. The low cytotoxicity of diamond nanoparticles affirms their utilization as biologically compatible materials.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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