Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of biosynthesis iron oxide nanoparticles by UV-Vis Spectroscopy (CAT#: STEM-MB-0936-WXH)

Introduction

Iron oxide nanoparticles are iron oxide particles with diameters between about 1 and 100 nanometers. The two main forms are magnetite (Fe3O4) and its oxidized form maghemite (γ-Fe2O3).
Applications of iron oxide nanoparticles include terabit magnetic storage devices, catalysis, sensors, superparamagnetic relaxometry, high-sensitivity biomolecular magnetic resonance imaging, magnetic particle imaging, magnetic fluid hyperthermia, separation of biomolecules, and targeted drug and gene delivery for medical diagnosis and therapeutics. These applications require coating of the nanoparticles by agents such as long-chain fatty acids, alkyl-substituted amines, and diols. They have been used in formulations for supplementation.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
Advertisement