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Analysis of BMPR1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2057-LGZ)

Introduction

Official Full Name: bone morphogenetic protein receptor type 1A<br />Also known as: ALK3; SKR5; ALK-3; CD292; ACVRLK3; BMPR-1A; 10q23del<br />Bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors ACVR1 and ACVR2. The ligands for these receptors are members of the TGF-β superfamily. TGF-β and activin signal by forming a heterocomplex with two different types of serine (threonine) kinase receptors: a type I receptor of about 50-55 kd and a type II receptor of about 70-80 kd. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling and type I receptors require their respective type II receptors for ligand binding.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements