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Analysis of CA15-3 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0071-LGZ)

Introduction

CA15-3 is the most important specific marker of breast cancer. 30%-50% of breast cancer patients have significantly increased CA15-3, and the change of its content is closely related to the treatment effect. It is the best indicator for breast cancer patients to diagnose and monitor postoperative recurrence and observe the curative effect. Dynamic determination of CA15-3 is helpful for early detection of recurrence in patients with stage II and stage III breast cancer after treatment; when CA15-3 is greater than 100U/ml, metastatic lesions can be considered.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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