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Analysis of CD14 (Human) by ELISA (CAT#: STEM-MB-0719-LGZ)

Introduction

The human CD14 gene is located on chromosome 5 at locus 5q23-31. According to the cDNA sequence of CD14, CD14 is composed of 356 amino acids. After the ribosome synthesizes the polypeptide, it is transported to the Golgi complex for glycosylation reaction, so CD14 is a glycoprotein. It was initially confirmed that CD14 is a myeloid differentiation antigen that exists in mature myeloid cells, while immature myeloid cells do not have CD14.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or cell culture supernatant
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