Analysis of CD1C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0829-LGZ)

Introduction

Official Full Name: CD1c molecule
Also known as: R7; CD1; CD1A; BDCA1
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of HHAT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1047-LGZ)
Analysis of OR56A5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2381-LGZ)
Analysis of NOTCH2 Gene (Mutation) in Hereditary Breast Ovarian Cancer Syndrome by RT-qPCR (CAT#: STEM-MT-0134-LGZ)
Analysis of Zika (ZIKV) and Chikungunya (CHIKV) Viruses by RT-qPCR (CAT#: STEM-MT-0065-LGZ)
Analysis of Fowl Adenovirus (FAdV-A) by Real-Time PCR Method (CAT#: STEM-MB-3791-LGZ)
Analysis of CENPS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1283-LGZ)
Analysis of USP40 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1778-LGZ)
Analysis of Carbapenem-resistant Klebsiella pneumoniae(R-Kp) by Real-Time PCR Method (CAT#: STEM-MB-2679-LGZ)