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Analysis of CD31/PECAM-1 (Human) by ELISA (CAT#: STEM-MB-0726-LGZ)

Introduction

CD31 is also known as Platelet endothelial cell adhesion molecule-1 (PECAM-1), with a molecular weight of 130kDa. The structure of CD31 belongs to the immunoglobulin superfamily and plays an important role in clearing aging neutrophils from the body. CD31 is found on the surface of platelets, neutrophils, monocytes, and some types of T cells, as well as in tight junctions between endothelial cells. It may be involved in leukocyte migration, angiogenesis and integrin activation.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or cell culture supernatant
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