Analysis of FGF-19 (Human) by ELISA (CAT#: STEM-MB-0811-LGZ)

Introduction

Fibroblast growth factor 19 (FGF-19) is encoded by the FGF-19 gene in humans. FGF-19 regulates bile acid synthesis and inhibits CYP7A1 through the FGFR4/Klotho-β receptor complex in the liver. In mouse models, FGF-19 also has metabolic effects, affecting glucose and lipid metabolism. Decreased FGF-19 in patients with chronic diarrhea due to bile acid malabsorption. FGF-19 is also present in the liver of cholestasis patients. FGF-19 expression is reduced in patients with metabolic syndrome, nonalcoholic fatty liver disease, and insulin resistance.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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