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Analysis of ApoE (Human) by ELISA (CAT#: STEM-MB-0672-LGZ)

Introduction

Apolipoprotein E (APOE), a secreted protein synthesized by liver cells and brain tissues, binds to a variety of receptors on the surface of different cells and plays a key role in lipid metabolism, immune regulation and neurology. ApoE is a polymorphic protein involved in the transformation and metabolism of lipoprotein, and its genes can regulate many biological functions, which is related to the pathogenesis of many eye diseases. The research on APOE and its gene polymorphism is one of the hot spots in medical research, and the exploration of the internal relationship between the two has important clinical application value for the prevention, diagnosis and treatment of eye diseases.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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