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Analysis of OX40L/TNFSF4 (Human) by ELISA (CAT#: STEM-MB-1111-LGZ)

Introduction

OX40 binds to the ligand OX40L (CD252, TNFSF4) to transmit co-stimulatory signals. The OX40 gene is located on chromosome 1 in humans and mice and encodes a 34kD transmembrane glycoprotein type II. OX40L can be expressed on antigen-presenting cells (APCs), such as B cells, dendritic cells and macrophages. In addition, expression can be induced in other cell types such as Langerhans cells, endothelial cells, smooth muscle cells, mast cells, and NK cells.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation, Immunogenicity

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
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