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All cells release membrane-coated vesicles in their environment, collectively known as extracellular vesicles (EV). These EVs contain a selected set of lipids, nucleic acids, and proteins that are derived from the cells of their origin and can therefore transmit a complex set of messages to surrounding or distant cells. Several transmembrane proteins, CD63, CD81, and CD9, have been used as exosome markers because of their accumulation in small EVs and steady-state accumulation of CD63 in MVB compared to whole-cell lysates.