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Analysis of CD81 (EV) (Human) by ELISA (CAT#: STEM-MB-0735-LGZ)

Introduction

All cells release membrane-coated vesicles in their environment, collectively known as extracellular vesicles (EV). These EVs contain a selected set of lipids, nucleic acids, and proteins that are derived from the cells of their origin and can therefore transmit a complex set of messages to surrounding or distant cells. Several transmembrane proteins, CD63, CD81, and CD9, have been used as exosome markers because of their accumulation in small EVs and steady-state accumulation of CD63 in MVB compared to whole-cell lysates.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or cell culture supernatant
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