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Analysis of CD9 (EV) (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0098-LGZ)

Introduction

All cells release membrane-coated vesicles in their environment, collectively known as extracellular vesicles (EV). These EVs contain a selected set of lipids, nucleic acids, and proteins that are derived from the cells of their origin and can therefore transmit a complex set of messages to surrounding or distant cells. Several transmembrane proteins, CD63, CD81, and CD9, have been used as exosome markers because of their accumulation in small EVs and steady-state accumulation of CD63 in MVB compared to whole-cell lysates.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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