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Analysis of CDC42BPA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0851-LGZ)

Introduction

Official Full Name: CDC42 binding protein kinase alpha
Also known as: MRCK; MRCKA; PK428
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interacting binding (CRIB) domain and has been shown to bind CDC42. It may act as a downstream effector of CDC42, mediating CDC42-induced peripheral actin formation and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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