Analysis of CELF3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0849-LGZ)

Introduction

Official Full Name: CUGBP Elav-like family member 3
Also known as: CAGH4; ERDA4; ETR-1; TNRC4; BRUNOL1
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of CATSPERE Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0838-LGZ)
Analysis of HNRNPUL2-BSCL2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2453-LGZ)
Analysis of CHD1L Gene (Mutation) in Hereditary Breast Ovarian Cancer Syndrome by RT-qPCR (CAT#: STEM-MT-0136-LGZ)
Analysis of LCE3C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0411-LGZ)
Analysis of Plasmodium Vivax by Real-Time PCR Method (CAT#: STEM-MB-2557-LGZ)
Analysis of Porcine Adenovirus (Swine Adenovirus) by Real-Time PCR Method (CAT#: STEM-MB-3186-LGZ)
Analysis of Porcine Circovirus 3 (PCV-3) by Real-Time PCR Method (CAT#: STEM-MB-3540-LGZ)
Analysis of PBXIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0562-LGZ)