Analysis of Chromogranin-A (Human) by ELISA (CAT#: STEM-MB-0743-LGZ)

Introduction

Chromogranin A (chromograninA, CGA) is a 48 kD acidic, hydrophilic secreted protein located in the chromaffin granules of neuroendocrine cells. CGA belongs to the chromaffin protein family and is present in catechol-secreting vesicles in all neuroendocrine cells, and was originally found in the secretory granules of adrenal chromaffin cells. In the chromaffin granules of the adrenal medulla, it is co-secreted with catecholamines and calcium. The protein is found in sympathetic nerve endings and myocardium, pancreas, central and peripheral nervous systems, intestinal endocrine tissues, thyroid and parathyroid glands and other tissues. Its proteolysis is tissue-specific, and the difference in the location of the break is related to the tissue in which it is located.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum

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