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Analysis of CTGF by ELISA (CAT#: STEM-MB-1534-LGZ)

Introduction

Connective tissue growth factor (CTGF) is a cell matrix protein in the CNN family. The extracellular portion of CTGF consists of four conserved cysteine-rich domains: insulin-like growth factor binding protein (IGFBP), von Willebrand type C repeat (vWC), thrombocystein-type 1 repeat (TSR), and cysteine-containing C-terminal domain (CT). CTGF plays an important role in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, bone development, and tissue wound repair. Monitoring serum levels of CTGF can provide more detailed insight into pathologic conditions such as malignant neoplasms, diabetic nephropathy, retinopathy, fibrosis, arthritis, pancreatic cancer and cardiovascular disease.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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