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Analysis of cIAP1 (Human) by ELISA (CAT#: STEM-MB-0744-LGZ)

Introduction

The inhibitor of apoptosis protein (IAP) family comprises a group of evolutionarily conserved inhibitors of apoptosis that contain a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain. Human protein members of this family include c-IAP1, c-IAP2, XIAP, surviving, livin, and NAIP. Members of the IAP family are overexpressed in cancer cell lines and primary tumors, and these proteins play an important role in cancer progression.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, tissue homogenate
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