Analysis of common ALK gene rearrangement in anaplastic large cell lymphoma (ALCL) by Fluorescence in situ hybridisation (FISH) (CAT#: STEM-MB-1220-WXH)

Introduction

The most common recurrent genetic aberration in anaplastic large cell lymphoma (ALCL) is translocation involving the ALK gene that results in ectopic expression of ALK protein in lymphoid tissue.
Anaplastic large cell lymphoma (ALCL) is a rare type of T-cell lymphoma. ALCL can occur at any age, but it is more common in children and young adults. It affects more men than women. Rarely, ALCL can develop in the scar tissue around a breast implant.

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Principle Applications Procedure Materials Notes Related Products

Principle

FISH uses fluorescent probes with complementary base sequences to locate the presence or absence of specific portions of DNA on chromosomes. The probe and target DNA must be denatured with heat or chemicals to break hydrogen bonds in the DNA and to allow hybridisation to occur once the two samples are mixed. The fluorescent probes form new hydrogen bonds with their complementary base pairs on the DNA, and these can then be detected via microscopy.

Applications

Detect and localize the presence or absence of specific DNA sequences on chromosomes.
Detect and localize specific RNA targets (mRNA, lncRNA and miRNA) in cells, circulating tumor cells, and tissue samples.

Procedure

1. Sample preparation
2. Co-denaturation and hybridization
3. Probe detection
4. Wash off of unbound probe
5. Analysis by flow cytometer/fluorescence microscopy

Materials

• Flow cytometer
• Fluorescence microscopy

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