Analysis of CXCL12/SDF-1 by ELISA (CAT#: STEM-MB-1591-LGZ)

Introduction

CXC ligand 12(CXCL12), also known as stromal cell derived factor 1(SDF-1), is a chemokine protein that activates leukocytes and is usually induced by pro-inflammatory stimulants such as LPS, TNF, or IL-1. CXCL12 showed strong chemotaxis to lymphocytes. During embryogenesis, it promotes hematopoietic cell migration from fetal liver to bone marrow and the formation of large blood vessels. In adults, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) in bone marrow through a CXCr4-dependent mechanism. CXCL12 also has an effect on tumor metastasis, and cancer cells expressing the CXCR4 receptor can be attracted to target tissues. However, in breast cancer, elevated CXCL12 expression suggests a reduced risk of distal metastasis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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