Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of CYB5R2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2226-LGZ)

Introduction

Official Full Name: cytochrome b5 reductase 2<br />Also known as: B5R.2<br />The protein encoded by this gene belongs to the yellow protein pyridine nucleotide cytochrome reductase protein family. Cytochrome b-type NAD(P)H oxidoreductases are involved in many processes such as cholesterol biosynthesis, fatty acid desaturation and elongation, and respiratory burst in neutrophils and macrophages. Cytochrome b5 reductase is available in soluble and membrane-bound forms and is a product of alternative splicing. In animal cells, the membrane-bound form is associated with the endoplasmic reticulum, where it is a member of the fatty acid desaturation complex. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements