Analysis of DIPK1A Gene (Mutation) in Diamond-Blackfan anemia by RT-qPCR (CAT#: STEM-MT-0117-LGZ)

Introduction

Also known as: FAM69A<br />This gene encodes a member of the FAM69 family of cysteine-rich type II transmembrane proteins. These proteins localize to the endoplasmic reticulum but their specific functions are unknown. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Diamond-Blackfan anemia

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of AARD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1908-LGZ)
Analysis of Infectious Hematopoietic Necrosis Virus (IHNV) by Real-Time PCR Method (CAT#: STEM-MB-3681-LGZ)
Analysis of OR52N5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2360-LGZ)
Analysis of Avian Infectious Bronchitis Virus Delaware Type (AIBV-Delaware) by Real-Time PCR Method (CAT#: STEM-MB-2859-LGZ)
Analysis of Porcine Rotavirus Group A by Real-Time PCR Method (CAT#: STEM-MB-3332-LGZ)
Analysis of Treponema Vincentii by Real-Time PCR Method (CAT#: STEM-MB-3194-LGZ)
Analysis of Vaccinia Virus (VV) by Real-Time PCR Method (CAT#: STEM-MB-4031-LGZ)
Analysis of Infectious Bursa Disease Virus (IBDV) by Real-Time PCR Method (CAT#: STEM-MB-3701-LGZ)